Dr. Brogden joined the College of Dentistry in 2004. He is director of the Iowa Institute for Oral Health Research and a professor in the Department of Periodontics. From October-January 2015, Dr. Brogden was also interim associate dean for research.
2004-present. Professor in the Department of Periodontics and Iowa Institute for Oral Health, College of Dentistry, The University of Iowa, Iowa City, IA 52242.
2003-2004. Research Program Representative for the Ames Modernization Plan, U.S. Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010. The Ames Modernization Plan was a $466.7 million dollar construction project to build the National Centers for Animal Health in Ames.
1999-2004. Research Leader (Department Head), Respiratory Diseases of Livestock Research Unit, U.S. Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010. The RDLRU consisted of three projects created to solve bacterial diseases of the respiratory tract of poultry, swine, and cattle, and had a staff of 11 scientists, 14 technicians, 3 students, and 1 secretary.
1999-2004. Collaborating Professor in the Cellular and Molecular Pathology specialty of Veterinary Pathology, Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011.
2001-2001. National Program Leader for Animal Health, National Program Staff, U.S. Department of Agriculture, Agricultural Research Service, George Washington Carver Center, 5601 Sunnyside Avenue, Beltsville, MD 20705. Served a 60-day detail from July 15 to September 15, 2001.
1981-1999. Category I Microbiologist, Respiratory and Neurologic Disease Research Unit, U.S. Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010.
Dr. Brogden's research focuses on the ability of peptides in saliva (defensins, histatins, etc.) to serve as downstream suppressors of cytokine signaling to recombinant hemagglutinin B (HagB) from the periodontal pathogen Porphyromonas gingivalis. In his work, they have found that human β defensin 3 (HBD3) has the capacity to influence the chemokine and cytokine responses of human dendritic cells: the timing of HBD3 exposure and the concentration of HBD3 treaatment of dendritic cells were both important in the onset, magnitude, and composition of the dendritic cell response to HagB. The mechanism may involve binding of HBD3 to HagB: HBD3 binds to immobilized HagB via surface plasmon spectroscopy and via ELISA and HBD3 inhibits binding of rHagB via surface plasmon spectroscopy and via ELISA and HBD3 inhibits binding of rHagB to cells via confocal microscopy and immunoelectron microscopy. Future research will determine the extent to which defensins can suppress early events in inflammation, an exciting concept that could be exploited to develop therapeutics to prevent or treat a variety of oral mucosal infections, particularly where inflammation plays a role in the pathogenesis of disease and its long-term sequelae.
Books
Squier, C., and Brogden, K. A. Human Oral Mucosa: Development, Structure, and Function. Wiley-Blackwell, West Sussex, UK. 168 pages. 2011.
Edited Books
Roth, J. A., Bolin, C. A., Brogden, K. A., Minion, F. C., and Wannemuehler, M. J. (eds). Virulence Mechanisms of Bacterial Pathogens, 2nd edition. ASM Press, Washington, D.C. 1995.
Brogden, K. A., Roth, J. A., Stanton, T. B., Bolin, C. A., Minion, F. C., and Wannemuehler, M. J. (eds). Virulence Mechanisms of Bacterial Pathogens, 3rd edition. ASM Press, Washington, D.C. 2000.
Brogden, K. A. and Guthmiller, J. M. (eds). Polymicrobial Diseases. ASM Press, Washington, D.C. 2002.
Brogden, K. A., Stanton, T. B., Cornick, N., Minion, F. C., Zhang, Q., Nolan L., and Wannemuehler, M. (eds). Virulence Mechanisms of Bacterial Pathogens, 4th Edition. ASM Press, Washington, D.C. 2007.
Fratamico, P. M., Smith, J. L., and Brogden, K. A. (eds). Post-Infectious Sequelae and Long-Term Consequences of Infectious Diseases. ASM Press, Washington, D.C. 2009.
Book Chapters
Parsons, N. J., Patel, P. V., Martin, P. M. V., Tan, E. L., Nairn, C. A., Goldner, M., Brogden, K. A., and Smith, H. Gonococci in vivo and in vitro. Further studies on the host and bacterial determinants of gonococcal resistance to killing by human serum and by phagocytes. In: Poolman, J. T., et al, (eds.). Gonococci and Meningococci. Kluwer Academic Publishers, Dordrecht. pp. 775-779. 1988.
Brogden, K. A. Pasteurella anatipestifer infection. In: Adlam, C. and Rutter, J. M. (eds.). Pasteurella and Pasteurellosis. Academic Press, London. pp. 115-129. 1989.
Phillips, M. and Brogden, K. A. Ultracentrifugation as a means for the separation and identification of lipopolysaccharides. In: Downstream Processing and Bioseparation. American Chemical Society Symposium Series 419:238-249. 1990.
Jacks, T. J., De Lucca II, A. J., and Brogden, K. A. Interaction of lipopolysaccharide with the antimicrobial peptide "cecropin A." In: Singh, B. R. and Tu, A. T. (eds). Natural Toxins II. Plenum Press, New York. pp. 357-360. 1996.
Brogden, K. A. Bacterial evasion of host-derived antimicrobial peptides on mucosal surfaces. In: Brogden, K. A., et al. (eds). Virulence Mechanisms of Bacterial Pathogens, 3rd ed. ASM Press, Washington, D.C. pp. 19-40. 2000.
Brogden, K. A. Polymicrobial diseases of animals and humans. In: Brogden, K. A. and Guthmiller, J. M. (eds). Polymicrobial Diseases. ASM Press, Washington, D.C. pp. 3-20. 2002.
Drake, D. R. and Brogden, K. A. Continuous culture chemostat systems and flowcells as methods to investigate microbial interactions. In: Brogden, K. A. and Guthmiller, J. M. (eds). Polymicrobial Diseases. ASM Press, Washington, D.C. pp. 21-30. 2002.
Brogden, K. A. and Guthmiller, J. M. Polymicrobial diseases: current and future research. In: Brogden, K. A. and Guthmiller, J. M. (eds). Polymicrobial Diseases. ASM Press, Washington, D.C. pp. 403-410. 2002.
Brogden, K. A., Ackermann, M., Zabner J., and Welsh, M. J. Antimicrobial peptides suppress microbial infections and sepsis in animal models. In: Mammalian Antimicrobial Peptides, Advances in Molecular and Cellular Microbiology Series. Devine, D. D. and Hancock, R. E. W. (eds). Cambridge University Press, pp. 189-228, 2004.
Pingel, L., Lu, X., and Brogden, K. A. Antimicrobial peptides as mucosal adjuvants. In: Brogden, K. A., Stanton, T. B.,Cornick, N., Minion, F. C., Zhang, Q, Nolan, L., Wannemuehler, M. (eds). Virulence Mechanisms of Bacterial Pathogens, 4th edition. ASM Press, Washington, D.C. pp. 281-295. 2007.
Brogden, K. A. Cytopathology of pathogenic prokaryotes. In Cheville, N. (ed). Ultrastructural Pathology: the Cellular Basis of Disease, 2nd edition. Blackwell Publishers, Ames, IA. pp. 425-523. 2009.
Joly, S. A., Belanger, M., Johnson, G. K., Progulske-Fox, A., and Brogden, K. A. Infection with Porphyromonas gingivalis, a potential risk factor for chornic systemic disease. In: Fratamico, P. M., Smith, J. L., and Brogden, K. A. (eds). Post-Infectious Sequelae and Long-Term Consequences of Infectious Diseases. ASM Press, Washington, D.C. pp. 443-457. 2009.
Fratamico, P. M., Smith, J. L., and Brogden, K. A. Concluding perspectives of sequelae and long-term consequences of infectious diseases -- what's next? In: Fratamico, P. M., Smith, J. L., and Brogden, K. A. (eds). Post-Infectious Sequelae and Long-Term Consequences of Infectious Diseases. ASM Press, Washington, D.C. pp. 487-493, 2009.
Bratt, C. L., Wertz, P., Drake, D., Dawson, D. V., and Brogden, K. A. Antimicrobial lipids of the skin and tear film. In: H. Thormar, H. Lipids and Essential Oils as Antimicrobial Agents. John Wiley & Sons, Ltd., Chichester, UK. 2010.
Brogden, K. A. Chapter 6. Perspectives and peptides of the next generation. In: Rebuffat, S. and Drider, D. (eds). Procaryotic Antimicrobial Peptides from Genes to Applications. Springer-USA. 2011
Brogden, K. A., Drake, D. R., Dawson, D. R., Hill, J. R., Bratt, C. L., and Wertz, P. W. Antimicrobial lipids of the skin and oral mucosa. In: Dayan, N. and Wertz, P. (eds.) Innate Immune System of Skin and Oral Mucosa: Properties and Impact in Pharmaceutics, Cosmetics, and Personal Care Products. Hoboken, NJ: John Wiley & Sons, Inc. pp. 75-81. 2011.
Journals
Brogden, K. A., De Lucca, A. J., Bland, J, and Elliott, S. Isolation of an ovine pulmonary surfactant-associated anionic peptide bactericidal for Pasteurella haemolytica. Proc Natl Acad Sci U.S.A. 93:412-416, 1996.
Brogden, K. A., Ackermann, M., McCray, P. B. and Tack, B. F. Antimicrobial peptides in animals and their role in host defences. Int J Antimicrob Agents. 22:465-478, 2003.
Brogden, K. A., Guthmiller, J. M. and Taylor, C. E. Human polymicrobial infections. Lancet. 365:253-255, 2005.
Brogden, K. A. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nature Reviews Microbiol. 3:1-13, 2005.
- The above paper was identified as a Fast Breaking Paper by Essential Science Indicators™ and as one of the most cited recent papers in the field of microbiology. Essential Science Indicators from Thomson Reuters lists highly cited papers in 22 broad fields of science. These papers comprise the top 1% of papers in each field and each year. Dr. Brogden was asked to provide comments on the paper, which in appeared in August 2006 on their website.
- The above paper was also included in the joint Nature Biotechnology and Nature Reviews Drug Discovery FOCUS on antibacterials. To view it, go to this website.
Brogden, K. A. Guthmiller JM. Salzet M. and Zasloff M. The nervous system and innate immunity: the neuropeptide connection. Nature Immunol. 6:558-564, 2005.
Shah, A. S., Farmen, S. L., Moninger, T. O., Businga, T. R., Andrews, M. P., Bugge, K., Searby, C. C., Nishimura, D., Brogden, K. A., Kline, J. N., Sheffield, V. C., Welsh, M. J. Loss of Bardet-Biedl syndrome proteins alters the morphology and function of motile cilia in airway epithelia. Proc Natl Acad Sci USA. 105:3380-3385, 2008. PubMed PMID: 18299575; PubMed Central PMCID: PMC2265193.
Rogers, C. S., Abraham, W. M., Brogden, K.A., Engelhardt, J. F., Fisher J., McCray Jr., P. B., McLennan, G., Meyerholz, D.K., Namati, E., Ostedgaard, L. S., Prather, R. S., Sabater, J. R., Stoltz, D. A., Zabner, J., and Welsh, M. J. The porcine lung as a potential model for cystic fibrosis. Am J Physiol Lung Cell Mol Physiol. 295:L240-263, 2008. PubMed PMID: 18487356; PubMed Central PMCID: PMC2519845.
Rogers, C. S., Stoltz, D. A., Meyerholz, D. K., Ostedgaard, K. S., Rokhlina, T., Taft, P. J., Rogan, M. P., Pezzulo, A. A., Karp, P. H., Itani, O. A., Kabel, A. C., Wohlford-Lenane, C. L., Davis, G. J., Smith, T. L., Samuel, M., Wax, D., Murphy, C. N., Rieke, A., Whitworth, K., Uc, A., Starner, T. D., Brogden, K. A., Shilyansky, J., McCray, P. B., Jr., Zabner, J., Prather, R. S., and Welsh, M. J. Disruption of the CFTR gene produces a model of cystic fibrosis in newborn piglets. Science. 321:1837-1841, 2008. PubMed PMID: 18818360; PubMed Central PMCID: MC2570747.
Pingel, L. C., Kohlgraf, K. G., Hansen, C. J., Eastman, C. G., Dietrich, D. E., Burnell, K. K., Srikantha, R. N., Xiao, X., Belanger, M., Progulske-Fox, A., Cavanaugh, J. E., Guthmiller, J. M., Johnson, G. K., Joly, S., Kurago, Z. B., Dawson, D. V., and Brogden, K. A. Outstanding Observation. Human beta-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesion from Porphyromonas gingivalis and attenuates a pro-inflammatory cytokine response. Immunol Cell Biol. 86:643-649, 2008. PubMed PMID: 18711400. [This article was the topics of the News and Commentary by McGillivary, G., and Bakaletz, L. O. Innate immune defenses -- finding a new way to calm the storm. Immunol Cell Biol 86:639-640, 2008.
Dietrich D. E., Xiano, X., Dawson, D. V., Belanger, M., Xie, H., Progulske-Fox, A., and Brogden, K. A. Human α- and β-defensins bind to immobilized adhesions from Porphyromonas gingivalis. Infect Immunol. 76:5714-5720, 2008. PubMed PMID: 18852241; PubMed Central PMCID: PMC2583589.
Kohlgraf, K. G., Ackermann, A., Lu, X., Burnell, K., Belanger, M., Cavanaugh, J. E., Xie, H., Progulske-Fox, A., and Brogden, K. A. Defensins attenuate cytokine responses yet enhance antibody responses to Porphyromonas gingivalis adhesins in mice. Future Microbiol. 5:115-125, 2010. PubMed PMID: 20020832; PubMed Central PMCID:PMC2822342.
Kohlgraf, K. G., Pingel, L. C., Dietrich, D. E., and Brogden, K. A. Defensins as anti-inflammatory compounds and mucosal adjuvants. Future Microbiol. 5:99-113, 2010. Review. PubMed PMID: 20020832; PubMed Central PMCID:PMC2820878.
Stoltz, D. A., Meyerholz, D. K., Pezzulo, A. A., Ramachandran, S., Rogan, M. P., Davis, G. JU., Hanfland, R. A., Wohlford-Lenane, C., Dohrn, C. L., Bartlett, J. A., Nelson, G. A. T., Chang, E. H., Taft, P. J., Ludwig, P. S., Estin, M., Hornick, E. E., Launspach, J. L., Samuel, M., Rokhlina, T., Karp, P. H., Ostedgaard, L. S., Uc, A., Starner, T. D., Horswill, A. R., Brogden, K. A., Prather, R. S., Richter, S. S., Shilyansky, J., McCray, P. B., Jr., Zabner, J., and Welsh, M. J. Cystic fibrosis pigs develop lung disease and exhibit defective bacterial eradication at birth. Sci Transl Med. 2:29ra31. PubMed PMID:20427821; PubMed Central PMCID:PMC2889616.
Brogden, N. K., and Brogden, K. A. Will new generations of modified antimicrobial peptides improve their potential as pharmaceuticals? Int J Antimicrob Agents. 38:217-25, 2011. PubMed PMID:21733662; PubMed Central PMCID: PMC3159164.
Fischer, C. L., Drake, D., Dawson, D. V., Blanchette, D. R., Brogden, K. A., Wertz, P. W. Antibacterial activity of sphingoid bases and fatty acids against gram-positive bacteria and gram-negative bacteria. Antimicrob Agents Chemother. 56:1157-1161, 2012.
Brogden, N. K., Mehalick, L., Fischer, C. L., Wertz, P. W., and Brogden, K. A. Review. The emerging role of peptides and lipids as antimicrobial epidermal barriers and modulators of local inflammation. Skin Pharmacol Physiol. 25:167-181, 2012.
Van Hemert, J. R., Recker, E. N., Dietrich, D., Progulske-Fox, A., Kurago, Z., Walters, K. S., Cavanaugh, J. E., and Brogden, K. A. Short Communicaation. HBD3 alters, but not inhibits, the binding of Porphyromonas gingivalis hemagglutinin B to the surface of human dendritic cells. J Antimicrob Agents. 40:75-79, 2012.
Fischer CL, Walters KS, Drake DR, Blanchette DR, Dawson DV, Brogden KA, Wertz PW. Sphingoid bases are taken up by Escherichia coli and Staphylococcus aureus and induce ultrastructural damage. Skin Pharmacol Physiol. 2013;26(1):36-44. doi: 10.1159/000343175. Epub 2012 Oct 30. PubMed PMID: 23128426; PubMed Central PMCID: PMC3634627.
Dawson DV, Drake DR, Hill JR, Brogden KA, Fischer CL, Wertz PW. Organization, barrier function and antimicrobial lipids of the oral mucosa. Int J Cosmet Sci. 2013 Jun;35(3):220-3. doi: 10.1111/ics.12038. Epub 2013 Feb 9. Review. PubMed PMID: 23320785; PubMed Central PMCID: PMC3640763.
Brogden KA, Johnson GK, Vincent SD, Abbasi T, Vali S. Oral inflammation, a role for antimicrobial peptide modulation of cytokine and chemokine responses. Expert Rev Anti Infect Ther. 2013 Oct;11(10):1097-113. doi: 10.1586/14787210.2013.836059. Review. PubMed PMID: 24124799.
Harvey LE, Kohlgraf KG, Mehalick LA, Raina M, Recker EN, Radhakrishnan S, Prasad SA, Vidva R, Progulske-Fox A, Cavanaugh JE, Vali S, Brogden KA. Defensin DEFB103 bidirectionally regulates chemokine and cytokine responses to a pro-inflammatory stimulus. Sci Rep. 2013;3:1232. doi: 10.1038/srep01232. Epub 2013 Feb 6. PubMed PMID: 23390582; PubMed Central PMCID: PMC3565171.
Fischer CL, Blanchette DR, Brogden KA, Dawson DV, Drake DR, Hill JR, Wertz PW. The roles of cutaneous lipids in host defense. Biochim Biophys Acta. 2014 Mar;1841(3):319-22. doi: 10.1016/j.bbalip.2013.08.012. Epub 2013 Aug 29. Review. PubMed PMID: 23994607; PubMed Central PMCID: PMC3943473.
Fischer CL, Walters KS, Drake DR, Dawson DV, Blanchette DR, Brogden KA, Wertz PW. Oral mucosal lipids are antibacterial against Porphyromonas gingivalis, induce ultrastructural damage, and alter bacterial lipid and protein compositions. Int J Oral Sci. 2013 Sep;5(3):130-40. doi: 10.1038/ijos.2013.28. Epub 2013 Jul 19. PubMed PMID: 23867843; PubMed Central PMCID: PMC3967327.
Holdren GO, Rosenthal DJ, Yang J, Bates AM, Fischer CL, Zhang Y, Brogden NK, Brogden KA. Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms. Antibiotics (Basel). 2014 Oct 23;3(4):527-539. PubMed PMID: 25859394; PubMed Central PMCID: PMC4387564.
Elangovan S, Brogden KA, Dawson DV, Blanchette D, Pagan-Rivera K, Stanford CM, Johnson GK, Recker E, Bowers R, Haynes WG, Avila-Ortiz G. Body fat indices and biomarkers of inflammation: a cross-sectional study with implications for obesity and peri-implant oral health. Int J Oral Maxillofac Implants. 2014 Nov-Dec;29(6):1429-34. doi: 10.11607/jomi.3758. PubMed PMID: 25397806.
Recker EN, Avila-Ortiz G, Fischer CL, Pagan-Rivera K, Brogden KA, Dawson DV, Elangovan S. A cross-sectional assessment of biomarker levels around implants versus natural teeth in periodontal maintenance patients. J Periodontol. 2015 Feb;86(2):264-72. doi: 10.1902/jop.2014.140354. Epub 2014 Sep 30. PubMed PMID: 25269523; PubMed Central PMCID: PMC4394381.
Dietrich DE, Martin AD, Brogden KA. Human β-defensin HBD3 binds to immobilized Bla g2 from the German cockroach (Blattella germanica). Peptides. 2014 Mar;53:265-9. doi: 10.1016/j.peptides.2014.01.017. Epub 2014 Feb 2. PubMed PMID: 24495736; PubMed Central PMCID: PMC3992933.
Borgwardt DS, Martin AD, Van Hemert JR, Yang J, Fischer CL, Recker EN, Nair PR, Vidva R, Chandrashekaraiah S, Progulske-Fox A, Drake D, Cavanaugh JE, Vali S, Zhang Y, Brogden KA. Histatin 5 binds to Porphyromonas gingivalis hemagglutinin B (HagB) and alters HagB-induced chemokine responses. Sci Rep. 2014 Jan 29;4:3904. doi: 10.1038/srep03904. PubMed PMID: 24473528; PubMed Central PMCID: PMC3912440.
Cassel SL, Janczy JR, Bing X, Wilson SP, Olivier AK, Otero JE, Iwakura Y, Shayakhmetov DM, Bassuk AG, Abu-Amer Y, Brogden KA, Burns TL, Sutterwala FS, Ferguson PJ. Inflammasome-independent IL-1β mediates autoinflammatory disease in Pstpip2-deficient mice. Proc Natl Acad Sci U S A. 2014 Jan 21;111(3):1072-7. doi: 10.1073/pnas.1318685111. Epub 2014 Jan 6. PubMed PMID: 24395802; PubMed Central PMCID: PMC3903222.
Barwacz CA, Brogden KA, Stanford CM, Dawson DV, Recker EN, Blanchette D. Comparison of pro-inflammatory cytokines and bone metabolism mediators around titanium and zirconia dental implant abutments following a minimum of 6 months of clinical function. Clin Oral Implants Res. 2015 Apr;26(4):e35-41. doi: 10.1111/clr.12326. Epub 2014 Jan 13. PubMed PMID: 24417614; PubMed Central PMCID: PMC4096610.
Patents
Brogden, K. A. and Chedid, L. P.C. 0249.92 - Pasteurella haemolytica subunit vaccine containing capsular polysaccharide and muramyl dipeptide. U.S. Patent 5,462,735. October 31, 1995.
Brogden, K. A. and Chedid, L. P.C. 0249.92 - Pasteurella haemolytica subunit vaccine containing capsular polysaccharide and muramyl dipeptide. International PCT Application No. PCT/US94/06368.
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Dr. Brogden is a member of the American Society for Microbiology, a Fellow in the American Academy of Microbiology, and a member of Phi Zeta and the American Association for the Advancement of Science. He is on the Board of Scientific Reviewers for the Annals of Agricultural and Environmental Medicine (1992-present) and was on the Board for Infection and Immunity (1998-2000). He has co-edited three books: Virulence Mechanisms of Bacterial Pathogens, second and third editions (ASM Press, Washington, D.C. (1995 and 2000) and Polymicrobial Diseases (ASM Press, Washington, D.C., 2002).