Microbiology, Immunology, Inflammation, & Oral Cancer
The response of the lining of the mouth and associated skin to environmental factors, local and systemic disease, the aging process, and traumatic injury, is not well understood. Nevertheless, such responses have important implications for preventing or treating periodontal disease, oral cancer, and other soft tissue diseases. Investigating the chemical composition, structure, and permeability of this system of tissues will lead to a better understanding of the barrier function of these tissues as a defense against microorganisms and carcinogens. Studying the action of tobacco components and alcohol on the mouth lining will help better define the etiology of oral cancer and how tobacco use may contribute to periodontal disease. And understanding the interactions between the immune system and oral cancer will lead to better treatment strategies.
- Dr. Jeffrey Banas
- Dr. Banas's broad research interest is the understanding of the pathogenic mechanisms of bacteria. Much of his work has focused on the microbiology of dental caries. His lab has extensively studied how the glucan-binding proteins (GBPs) synthesized by Streptococcus mutans contribute to the development of the plaque biofilm and ultimately dental caries. Since glucan -- a polymer of glucose derived from the metabolism of sucrose -- is a primary virulence factor that propels the change in microbial ecology that leads to a cariogenic plaque, it has been proposed that proteins that have the property of binding glucan play accessory roles in this process. It is now clear that the GBPs share in structurally supporting the biofilm, each doing so in a unique manner. These studies also revealed that the relative coverage of bacteria at the substratum surface of the biofilm was correlated with the risk of caries development.
- Dr. Kim Brogden
- Dr. Brogden’s current area of research focuses on the ability of peptides in saliva (defensins, histatins, etc.) to serve as upstream suppressors of cytokine signaling to recombinant hemagglutinin B (rHagB) and recombinant Fimbrillin (rFimA) from the periodontal pathogen Porphyromonas gingivalis. Recently, human beta defensin 3 and histatin 5 were found to attenuate a pro-inflammatory cytokine response induced by rHagB in human myeloid dendritic cell culture supernatants and the Extracellular signal-Regulated Kinases (ERK 1/2) response induced by rHagB in human myeloid dendritic cell lysates. The mechanism may involve binding of HBD3 to rHagB and rFimA: HBD3 binds to immobilized rHagB and rFimA via surface plasmon spectroscopy and via ELISA and HBD3 inhibits binding of rHagB to cells via confocal microscopy and immunoelectron microscopy. Future research will determine the extent to which defensins can suppress early events in inflammation, an exciting concept that could be exploited to develop therapeutics to prevent or treat a variety of oral mucosal infections, particularly where inflammation plays a role in the pathogenesis of disease and its long-term sequelae.
- Dr. David R. Drake
- Dr. Drake's current research interests are focused on transmission of specific genotypes of Streptococcus mutans (SM) from mother to child in different populations. His current R01 study is looking at acquisition of SM in American Indian children from birth to three years of age. He is conducting this study in partnership with the Oglala Sioux Tribe in Pine Ridge, South Dakota. Dr. Drake has a field team consisting of dental hygienists and research assistants on site who collect plaque samples from mothers, primary caregivers, and the children. In addition, a comprehensive approach to obtain dietary and behavioral data, as well as oral health (caries assessment) is being done. The plaque samples are shipped via Federal Express to his laboratories here at the University of Iowa, where he and his research team are performing analyses on the microflora isolated from the clinical samples, with a focus on DNA fingerprinting of SM isolates. He is also involved in the R01 research on oral antimicrobial lipids led by Dr. Philip Wertz (PI) and in collaboration with Drs. Kim Brogden and Deborah Dawson.
- Dr. Georgia Johnson
- Effects of tobacco products on periodontal host response and the impact of smoking on periodontal treatment outcomes; the role of naturally occurring antimicrobial peptides in periodontal disease and clinical dental implant research.
- Dr. Arwa Owais
- Dr. Owais's research interests include prevention of dental caries in infants, toddlers, and adolescents, especially children of low income, limited access to care and high risk populations. Also of special interest to her is the medical management of caries in children, adolescents and adults. She is conducting research on silver compounds and their effect on preventing and arresting caries. Dr. Owais is a co-principal investigator of an ongoing clinical trial investigating medical management of dental caries in children using silver nitrate.
- Dr. Christopher Squier
- For over twenty-five years, Dr. Squier's research has focused on the structure and function of stratified squamous mucosae, with particular regard to the nature of the superficial permeability barrier structure and the role of topical carcinogens and micro-organisms in the pathogenesis of local and systemic disease. Specific areas of interest have been interactions between alcohol and tobacco components in the development of oral cancer and the mechanisms by which Staphylococcae and their toxins are involved in the vaginal pathogenesis of toxic shock syndrome. More recently, he has been concerned with the role of oral health professionals in assisting their patients with tobacco cessation. Dr. Squier has published more than 200 books, chapters, and peer-reviewed articles.
- Dr. Philip Wertz
- Biochemistry; structures, function and metabolism of lipids in epidermis and oral epithelium.